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Eastern Washington University
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Cheney, WA 99004
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Rita Miranda

1997 Research

Abstract: Determination of Pyruvate Stability in CZB Embryo Culture Media
Mentor: Dr. Charles Herr, Biology

In the metabolic process known as glycolysis, a molecule of glucose is degraded in a series of enzyme-catalyzed reactions to yield two molecules of pyruvate (pyruvic acid). In aerobic organisms or tissues, under aerobic conditions, glycolysis constitutes only the first stage in the complete degradation of glucose. Pyruvate is then oxidized to yield the acetyl group of acetyl coenzyme A, which is then oxidized completely to carbon dioxide by the citric acid cycle. The electrons from these oxidations are passed to oxygen through a chain of carriers in the mitochondrion, which forms water as its end product. The energy from the electron transfer reactions drives the synthesis of adenosine triphosphate (ATP) in the mitochondrion. ATP functions as the major carrier of chemical energy in all cells, and drives the reactions in the body necessary to sustain life.

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