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Eastern Washington University
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Gianna Hammer

1998 Research

Abstract: Creation of a Caprine Interleukin-10 Clone
Purpose: the future study of IL-10 in association with the Caprine Arthritis Encephalitis Virus (CAEV)
Mentor: Dr. William Cheevers, Biology

The development of a Caprine Interleukin-10 (IL-10) recombinant plasmid was facilitated through the use of PCR amplification using forward and reverse primers encoding the recognition sequence for enzymatic digestion with NDE I and BAMH I respectively. After direct ligation into an Easy Vector Plasmid and bacterial transformation, the plasmid was digested with the selected enzymes, and the DNA fragments were electrophoretically separated. A gel purified suspension of the insert was then ligated into a primed pET plasmid. Plasmids were isolated from bacterial cells transformed with the products of the ligation reaction. Enzymatic digestion and subsequent electrophoresis allowed for the identification of recombinant plasmids.

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