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Eastern Washington University
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Olga Shiva

2009 Research

Abstract: Identifying Promoters of Helicobacter pylori Genes in the Clinically Important Cytotoxin-Associated Gene Pathogenicity Island

Mentor: Dr.Andrea Castillo, Biology

Helicobacter pylori is a gram negative, helical shaped microareophilic bacterium that inhabits the stomach and duodenum of 50% of the world‟s population. Approximately 10% of infections result in gastric disease, including peptic and duodenal ulcers, mucosa-associated lymphoid tissue lymphoma and gastric adenocarcinoma. The more severe pathologies have been associated with the presence of the cytotoxin-associated gene pathogenicity island (cagPAI) in infecting H. pylori strains. Relatively little is known about how the ~30 cagPAI genes are regulated. In my study, I have amplified the 40kb cagPAI region as X PCR amplicons and begun cloning digested fragments of this region into a reporter plasmid. To functionally identify cagPAI gene promoters, I will transform this cagPAI fragment-reporter plasmid library into H. pylori and look for expression of my reporter, read as antibiotic sensitivity.

Presentations

19th Annual National McNair Research Conference and Graduate Fair, November 5-7, 2010

Present Summer 2010 research as a participant in the Fred Hutchinson Research Opportunity at U. Washington

13th Annual EWU Student Research and Creative Works Symposium: May 19, 2010

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